![]() Nucleotide excision repair-defective xeroderma pigmentosum cells were sensitized up to 1000-fold, implicating bulky DNA photoproducts in the lethal effect. ![]() 4-thiothymidine induced UVA dose enhancements of around 100-fold in DNA repair-proficient cells. It does not induce MMR-related toxicity, but it interacts synergistically with UVA light and dramatically sensitizes cultured human cells to very low, nonlethal UVA doses. We report here that the thiopyrimidine nucleoside 4-thiothymidine is incorporated into DNA. ![]() In addition to these direct cytotoxic effects, DNA thiobases have distinctive photochemical properties, the therapeutic potential of which has not been extensively evaluated. Their toxicity requires active DNA mismatch repair (MMR), and thiopurine resistance is an acknowledged phenotype of MMR-defective cells. The thiopurines, 6-thioguanine and 6-mercaptopurine, are antileukemic agents that are incorporated into DNA following retrieval by the purine salvage pathway (see for a review). Photoactivation of DNA thiobases as a potential novel therapeutic option. ![]() Massey, Andrew Xu, Yao-Zhong and Karran, Peter ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |